Islamic lifestyle with a focus on health

Islamic lifestyle with a focus on health

Effect of Four Weeks of Increasing Resistance Training with DatePalm Pollen Extract on the Gene expression of CHRN-G and Semaphorine-A3 in the Gastrocnemius Muscle of Healthy Male

Authors
1 Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Sports Sciences, Tehran East Branch, Islamic Azad University, Tehran, Iran
Abstract
Purpose: Regular exercise training with different volumes by
date palm pollen extract is an effectives strategy in improving
gene expression and neuromuscular function. The purpose of
this study was to determine the Effect of four weeks of
increasing resistance training with date palm pollen extract on
the gene expression of CHRN-G and Semaphorine-a3 in the
Gastrocnemius muscle of healthy male rats.
Materials and Methods: This is an experimental study. For
this purpose, 42 six week old healthy of male rats were divided
into 6 groups of numbers 7; Control (C), Resistance training
(TR), Testosterone (T), SUP (S), Resistance training with
extract (RT+SUP), Resistance training with Testostrone
(RT+T). Extraction was performed with 90% ethanol at a rate
of 670 ml and repeated three times. The extract was
concentrated in vacuo and the solvent was completely
removed. The extraction efficiency was 87.17%. Consumption
of the extract at a dose of 100 mg / kg per day to the extract
groups and exercise with the extract was gavaged
intraperitoneally. Testosterone at a dose of 2 mg / kg per day
was injected into the testosterone and testosterone training
groups.increasing resistance training, extract and testosterone
interventions were performed for five days a week for four
weeks. After 24 hours the last training session and afterward
recovery, subjects were sacrificed and their gastrocnemius
muscle was extracted. PCR Real time was used to determine
the expression of CHRN-G and Semaphorine-a3 genes. to
compare the groups independent Two way anova was used at
alpha level of 0/05.
Findings: genes expression of CHRN-G in the training with
extract group (P=0.024) and training with testosterone
(P=0.045) respectively showed a significant decrease
compared to the extract group. Semaphorine-a3 of gene in the
training with extract group (P=0.016) respectively showed a
significant decrease compared to the extract group. Weight did
not significantly change in any of the groups.
Conclusion: It seems that possibly 4 weeks of increasing
resistance training with consumption of date palm male pollen
extract can improve neuromuscular function by reducing the
expression of CHRN-G and Semaphorine-a3 genes in the
presynaptic space.
Keywords

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Journal of Islamic Life Style Volume 6, Winter 2022
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