pose: The purpose of this study was the effect of high intensity interval training with caloric restriction on rat myocardial apoptosis markers
Materials and Methods: 27 male rats were randomly divided into three groups: control, caloric restriction and caloric restriction with high-intensity interval training. During the study, the control group had free access to water and food, but the amount of food in the two groups was limited to 50% of the control group. The restriction and exercise group was exposed to intense interval training for eight weeks. Relative expression of caspase 3 and 9 Gen was obtained by real-time PCR. Data were analyzed by one-way ANOVA and Tukey's post hoc test.
Finding: The mean weight of rats in both groups of caloric restriction decreased significantly during the study (p=0.001). One-way analysis of variance showed a significant difference between the means of the three groups in the expression of caspase protein 3 and 9 in the heart muscle of male rats (p=0.001). The results of post hoc test showed a significant decrease in caspase 3 and 9 cardiac muscle expression in male rats in both groups of caloric restriction compared to the control group. Also, no significant difference was observed between the two groups of limitations (p = 0.102).
Conclusion: Dietary restriction seems to be associated with a reduction in fat mass as well as muscle mass, so combining intermittent exercise with food restriction may be effective in slowing the process of skeletal muscle protein synthesis.